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Image Search Results
Journal: Cancer Immunology, Immunotherapy : CII
Article Title: Activation of complement C3, C5, and C9 genes in tumors treated by photodynamic therapy
doi: 10.1007/s00262-006-0221-z
Figure Lengend Snippet: The expression of complement genes C3, C5, and C9 in tumors treated by PDT and the livers of host mice. Mice bearing subcutaneous LLC tumors were treated by PDT (Photofrin 10 mg/kg i.v. followed 24 h later by tumor-localized light dose of 150 J/cm2). Tumor and liver samples were collected from mice sacrificed at different times after PDT light treatment and used for the determination of the expression of investigated genes by RT-PCR. a Representative example of generated electrophoresis gels with bands of G (reference gene GAPDH), C3, C5, and C9 genes (composed using three individually run gels from the same experiment). The values obtained from band intensity measurement, that were first normalized for GAPDH expression in the same tissue and then calculated as ratios relative to the level of expression in non-treated control mice, are shown separately for C3 (b), C5 (c) and C9 gene (d). Bars represent SD (n = 4), asterisk denotes statistically significant difference (p < 0.05) compared to the pre-treatment levels in the respective control group
Article Snippet: PDT treatment The protocol for PDT started with the administration of
Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Generated, Electrophoresis, Control
Journal: Cancer Immunology, Immunotherapy : CII
Article Title: Activation of complement C3, C5, and C9 genes in tumors treated by photodynamic therapy
doi: 10.1007/s00262-006-0221-z
Figure Lengend Snippet: The effect of PDT treatment in vitro on the expression of C3, C5, and C9 genes in LLC tumor cells and TAMs. For PDT treatment, LLC cells or TAMs obtained from non-treated LLC tumors were incubated with Photofrin (20 μg/ml for 24 h) and then exposed to the light dose of 1 J/cm2 (LLC-PDT and TAMs-PDT, respectively). In addition, non-treated TAMs (TAMs-NT) were co-incubated with non-treated LLC (LLC-NT) or PDT-treated LLC cells contained in tissue culture inserts with a porous base. Following 8 h of incubation, the cells were harvested for the RT-PCR based analysis of the expression of genes of interest. The results are shown as GAPDH-normalized values relative to those obtained with respective PDT-non-treated control cells incubated alone. Bars represent SD (n = 4), asterisk denotes statistically significant difference (p < 0.05) compared to the values for the respective gene obtained with PDT-non-treated TAMs incubated alone
Article Snippet: PDT treatment The protocol for PDT started with the administration of
Techniques: In Vitro, Expressing, Incubation, Reverse Transcription Polymerase Chain Reaction, Control